The degree of hydrolysis in the samples was determined according to the L-serine equal strategy. The information ended up received from a few impartial hydrolyses for each roe and protease combination. Evaluation of variance (ANOVA) was performed. Letters a–c suggest important variations amongst unique samples prepared with various enzyme concent
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The 2 protein bands from 40 kDa to 30 kDa were being absent immediately after 24 h incubation at 45 °C. For the rest of the bands, various concentrations of all 3 proteases retained protein bands similar to their initial molar masses (Determine 6a,b). The presence of bands at low molecular pounds in fish roe in advance of hydrolysis was unanticipa
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